Experimental protein
Experimental proteinABSTRACT
Proteins are the macromolecules that are responsible for most of the bodily functions. By investigating an individual protein, one can be able to understand the functions and structure of an organism. Before this can be done, protein has to be separated from cell components. Using the methods of centrifugation and gel electrophoresis, not only a protein can be separated from cellular components but also from other proteins. An experiment was designed to separate protein contained in liver tissue of a cow based on it's molecular weight. From the centrifugation, soluble protein concentration extracted for the electrophoresis. Dilution was prepared of this concentrated extract; positive correlation made between the concentration of BSA and measured absorbance. This correlation helped to draft a regression line, which was useful in finding concentration of protein extract. An electrotransfer on PVDF membrane was done after the gel electrophoresis. These migrations of proteins based on their molecular weight of three solutions. Using the polyacrilamide electrophoresis, proteins were separated based on their molecular weight. Extraction buffer did not produce protein bands. Proteins with huge molecular weight did not travel far from the gel while low molecular weight proteins traveled long distance . Overall banding contrast was compared between same sample with different transferred volumes. The protein bands of ADH were closely related to some of the bands of liver protein extract. ADH, which is derived from horse contains some of the protein that are related to the proteins found on cow's liver cells. Closely ranged Rf value is key to similarities that exists between protein bands based on their molecular weight. The results of these experiments were compared with research group with similar interests; the results and conclusion drawn were closely correlated.
Introduction
Our body consists of various types of macromolecules, which are the fundamental functional unit of life. These macromolecules are responsible for most of all of the cell functions. They include molecules such as carbohydrates (glycogen, starch, cellulose, chitin), lipids (fats, fatty acids, steroids, phospholipids), nucleic acids (RNA, DNA, rRNA, mRNA, tRNA), and proteins (1,2).
These proteins are the molecules that regulate all of the bodily functions in an organism. Proteins provide structural, chemical, hormonal, and metabolic support an organism attain for everyday life. Proteins acts as transporters to synthesize genes, regulatory functions as found in membranes, receptors to specific ligands, and structural support found in muscles. All proteins are made from the monomers of amino acids (1,2).
The chain of amino acids is linked by a peptide bond; peptide bond in synthesized by peptidyl tranferace located in the large ribosomal unit of the ribosome. The shape or conformation determines the function of a protein. The shape is determined as protein's polypeptide chain is synthesized by the ribosome (1,2).
The structure of proteins is separated into four organizational level (primary, secondary, tertiary, quaternary); it...
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